Journal: Pharmaceutics

Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

doi: 10.3390/pharmaceutics17010138

Figure Lengend Snippet: Pro-collagen I ( A ) and elastin ( B ) levels measured by ELISA assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).

Article Snippet: Pro-collagen I, elastin, MMP-1, and TIMP-1 dosage were measured by using non-competitive sandwich ELISA (Human Pro-Collagen I α-1 Simple-Step kit ab210966, Abcam, Cambridge, UK; Human Elastin ELISA ab239433, Abcam; Human MMP-1 BMS2016-2, Afflimetrix-Thermofisher, Santa Clara, CA, USA; and Human TIMP-1 MA5-13688 (Afflimetrix-Thermofisher) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison

Journal: Pharmaceutics

Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

doi: 10.3390/pharmaceutics17010138

Figure Lengend Snippet: Analysis of MMP-1 ( A ) and TIMP-1 ( B ) protein levels by ELISA assay in HFF cell lysate after treatment with ZPP at the concentrations of 1, 10, and 100 µg/mL for 24 h. Data were analyzed using one-way ANOVA (MMP-1: F(3,7) = 0.340, p = 0.7978; TIMP-1: F(3,7) = 1.445, p = 0.3089) followed by Dunnett’s post hoc multiple comparison ( p > 0.05). Dosage of MMP-1 ( C ) and TIMP-1 ( D ) protein levels in HFF cell lysate after pre-treatment for 2 h with ZPP at the concentrations of 1, 10, and 100 µg/mL then exposed to MetPRED (100 µg/mL) for 6 h. One-way ANOVA (MMP-1: F(4,14) = 1.551, p = 0.2450; TIMP-1: F(4,13) = 2.795, p = 0.0709) followed by Tukey’s post hoc multiple comparison; (a) p < 0.05 vs. CTRL, (n = 6). Data are expressed as mean ± SD. Control cells (CTRL) were arbitrarily set to 1.

Article Snippet: Pro-collagen I, elastin, MMP-1, and TIMP-1 dosage were measured by using non-competitive sandwich ELISA (Human Pro-Collagen I α-1 Simple-Step kit ab210966, Abcam, Cambridge, UK; Human Elastin ELISA ab239433, Abcam; Human MMP-1 BMS2016-2, Afflimetrix-Thermofisher, Santa Clara, CA, USA; and Human TIMP-1 MA5-13688 (Afflimetrix-Thermofisher) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: Antioxidants (Basel, Switzerland)

Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.

doi: 10.3390/antiox11101881

Figure Lengend Snippet: Figure 9. Effects of UF and LCF on Nrf2/Keap1 signaling pathway. Effects of UF and LCF on Nrf2 content (a) and mRNA expression (b), and Keap1 content (c) and mRNA expression (d). Effects of UF and LCF on activity of NQO-1 (e) and mRNA expression (f), HO-1 content (g) and mRNA expression (h), and GCLC content (i) and mRNA expression (j). UF: unfermentation; LCF: single microbial fermentation with L. curvatus. Analysis of significant differences between each group and H2O2 model group, #: p < 0.05; ##: p < 0.01; ###: p < 0.001.; comparison of each group between UF and LCF, *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns: p > 0.05.

Article Snippet: PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China.

Techniques: Expressing, Activity Assay, Comparison

Journal: Antioxidants (Basel, Switzerland)

Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.

doi: 10.3390/antiox11101881

Figure Lengend Snippet: Figure 13. The oxidative stress pathway of Nrf2/Keap1/Mafk.

Article Snippet: PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China.

Techniques: